Re: track multiple ROIs movements

Posted by dpoburko on Apr 20, 2009; 5:20pm
URL: http://imagej.273.s1.nabble.com/Re-track-multiple-ROIs-movements-tp3692841.html

Hi All,

  As usual, I apologize is I missed some previous threads on this topic.

  I am trying to track multiple (50 - ~500) ROIs over time. Basically,
every few minutes, I grab a reference image that will be segmented to
define ROIs for extracting dynamic responses from a different channel
that is acquired with higher temporal resolution. So essentially, I need
a method to compare and register ROIs in reference images (in this case
a synaptic bouton) over time. At the moment, I am simply calculating a
minimum distance between ROI centroids in Excel, and excluding those
that are above a cut-off distance. I am wondering if anyone has
experience with or can suggest a more sophisticated method. For example,
something that takes into account the relative position of one ROI (or
centroid) in a constellation of numerous other centroids. Or perhaps
analyze the distance and direction to several nearest neighbors, and use
that a criteria to compare with the next image. My plan is eventually to
run this in a form of batch mode, so it doesn't have to be terribly fast.  

Many thanks,
Damon
--

Damon Poburko, PhD
Postdoctoral Research Fellow
Stanford University School of Medicine
Dept. of Molecular & Cellular Physiology
279 Campus Dr., Beckman B103, Stanford, CA 94305
Ph: 650 725 7564, fax: [phone]