Re: track multiple ROIs movements

Posted by David Webster on Apr 20, 2009; 6:10pm
URL: http://imagej.273.s1.nabble.com/Re-track-multiple-ROIs-movements-tp3692841p3692843.html

Damon,

I have done this type of point pattern matching (or alignment) using a
Generalized Hough Transform. For what it's worth, this is nothng like the
regular Hough Transform used to look for lines or circles.

David

On Mon, Apr 20, 2009 at 10:20 AM, Damon Poburko <[hidden email]>wrote:

> Hi All,
>
>  As usual, I apologize is I missed some previous threads on this topic.
>
>  I am trying to track multiple (50 - ~500) ROIs over time. Basically, every
> few minutes, I grab a reference image that will be segmented to define ROIs
> for extracting dynamic responses from a different channel that is acquired
> with higher temporal resolution. So essentially, I need a method to compare
> and register ROIs in reference images (in this case a synaptic bouton) over
> time. At the moment, I am simply calculating a minimum distance between ROI
> centroids in Excel, and excluding those that are above a cut-off distance. I
> am wondering if anyone has experience with or can suggest a more
> sophisticated method. For example, something that takes into account the
> relative position of one ROI (or centroid) in a constellation of numerous
> other centroids. Or perhaps analyze the distance and direction to several
> nearest neighbors, and use that a criteria to compare with the next image.
> My plan is eventually to run this in a form of batch mode, so it doesn't
> have to be terribly fast.
> Many thanks,
> Damon
> --
>
> Damon Poburko, PhD
> Postdoctoral Research Fellow
> Stanford University School of Medicine
> Dept. of Molecular & Cellular Physiology
> 279 Campus Dr., Beckman B103, Stanford, CA 94305
> Ph: 650 725 7564, fax: [phone]
>