Posted by
Fabrice Senger on
URL: http://imagej.273.s1.nabble.com/Disjoint-ROI-selection-using-macro-language-tp3692854p3692861.html
Ben Tupper a écrit :
> On Apr 17, 2009, at 4:08 AM, Senger Fabrice wrote:
>>>
>> Hi there,
>>
>> i'm sorry to not have the answer to your problem but I take the
>> opportunity to talk about a problem I have. I'm segmenting cells and
>> add them to the manager what I would do next is extract each cell to
>> generate a new image by using the corresponding ROI.
>> Did someone allready tried such an approach ?
>>
>> Thanks,
>>
>> Fabrice.
>>
>>
>
>
> Hi Fabrice,
>
> If you want to transfer a region from one image to another then you
> could do it with something like the following (note that ROI Manager
> is not used).
>
> //START MACRO
>
> run("Blobs (25K)");
> setAutoThreshold();
> //run("Threshold...");
> setThreshold(125, 255);
> run("Convert to Mask");
> maskID = getImageID();
> run("Set Measurements...", "area mean standard modal min centroid
> center perimeter bounding fit shape feret's integrated median skewness
> kurtosis redirect=None decimal=3");
> run("Analyze Particles...", "size=0-Infinity circularity=0.00-1.00
> show=Nothing display clear record ");
>
> //first method creates a new image to accept the subimage
> ix = 22;
> bx = getResult("BX", ix);
> by = getResult("BY", ix);
> w = getResult("Width", ix);
> h = getResult("Height", ix);
>
> name = "#" + ix+1;
> newImage(name, "8-bit White", w,h,1);
> img23ID = getImageID();
>
> selectImage(maskID);
> makeRectangle(bx,by,w,h);
> run("Copy");
> selectImage(img23ID);
> run("Paste");
> run("Invert");
>
>
> // the second method exploits the File > New > Internal Clipboard menu
> item
> ix = 30;
> bx = getResult("BX", ix);
> by = getResult("BY", ix);
> w = getResult("Width", ix);
> h = getResult("Height", ix);
>
> selectImage(maskID);
> makeRectangle(bx,by,w,h);
> run("Copy");
> name = "#" + ix+1;
> run("Internal Clipboard");
> rename(name);
>
> //END MACRO
>
>
>
> Ben Tupper
Hi,
your mail some how gives me a first answer but actually my question is
more like :
I have a picture with 10 cells in it.
I'm able to segment them.
Next for each cell I would create a new picture.
So I don't want to copy one specific particle, I would like to create
one image for each particle.
This is part of an analysis scheme where I plan to isolate cells from a
picture into a folder and then batch process this folder with another macro.
--
Senger Fabrice