Reslice stack with a rotated rectangular selection.

Posted by Michael Cundell on Apr 11, 2009; 6:32pm
URL: http://imagej.273.s1.nabble.com/Reslice-stack-with-a-rotated-rectangular-selection-tp3692983.html

Hey,

Is there a plugin that will let me use the reslice command with a rectangular selection that I have rotated using edit>selection>rotate.  When you rotate a rectangular selection reslice does not work anymore saying "line or rectangular selection required".

The object im reslicing needs to be horizontal, and then i perform reslice from the top on my stack.  I have about 50 cells im reslicing per field of view but all the cells are all at different angles.  Currently im having to rotate my image constantly using image>rotate, getting each object im reslicing within the cell horizontal, then reslicing from the top with a normal rectangular selection.  Im using interpolation when rotating my image.  Is using interpolation and rotating many times a bad thing? the object im looking at after reslicing is only about 1.5 microns in diameter and is relatively close to the microscopes Z-sectioning/camera resolution.

I should also say that i dont use imageJ extensively, and I do not know how to code, so if there is any options for what im trying to do Lehman's terms would be much appreciated.

Thanks !