Re: Reslice stack with a rotated rectangular selection.

Posted by Bill Mohler on Apr 11, 2009; 8:10pm
URL: http://imagej.273.s1.nabble.com/Reslice-stack-with-a-rotated-rectangular-selection-tp3692983p3692984.html

You should experiment with reslicing from a line selection. You can
start from any angle, and then define how far you want the slicing to
progress orthogonally.

>Hey,
>
>Is there a plugin that will let me use the reslice command with a
>rectangular selection that I have rotated using
>edit>selection>rotate.  When you rotate a rectangular selection
>reslice does not work anymore saying "line or rectangular selection
>required".
>
>The object im reslicing needs to be horizontal, and then i perform
>reslice from the top on my stack.  I have about 50 cells im
>reslicing per field of view but all the cells are all at different
>angles.  Currently im having to rotate my image constantly using
>image>rotate, getting each object im reslicing within the cell
>horizontal, then reslicing from the top with a normal rectangular
>selection.  Im using interpolation when rotating my image.  Is using
>interpolation and rotating many times a bad thing? the object im
>looking at after reslicing is only about 1.5 microns in diameter and
>is relatively close to the microscopes Z-sectioning/camera
>resolution.
>
>I should also say that i dont use imageJ extensively, and I do not
>know how to code, so if there is any options for what im trying to
>do Lehman's terms would be much appreciated.
>
>Thanks !

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William A. Mohler
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Dept. of Genetics and Developmental Biology
University of Connecticut Health Center
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