Re: regarding IHC PROBLEM

Posted by Santosh M on Apr 07, 2009; 2:39pm
URL: http://imagej.273.s1.nabble.com/regarding-IHC-PROBLEM-tp3693036p3693039.html

Thank you for your  reply
according to mailing list archive: DAB cannot be used for absorption
photometry, as it does not meet the Beer-Lambert criteria for a
phometric material.
(http://n2.nabble.com/Re--Nuclear-volume,-ploidy-and-Stain-stochiometry-td2347676.html)
But I feel Color Deconvolution plugin it is at least differentiating colours.
I have seen some articles in which they have used for  quantitative
immunohistochemistry for DAB stained slides softwares like Image pro
plus and Q-IHC.

So What is the best solution available for quantitative
immunohistochemistry for DAB stained slides?

Thanks for any suggestions

Santosh M



2009/4/7 Gabriel Landini <[hidden email]>:

>> Santosh M wrote:
>> > How can i quantity only leaving out Haematoxycillin ?
>> > its urgent plz
>
> On Tuesday 07 April 2009 11:37:41 Przemko wrote:
>> Use Color Deconvolution plugin. IT IS GREAT!!!!!!!!!!!!!
>
> You can separate the stains that way, but I you must be aware that DAB
> staining cannot be quantified reliably by its intensity.
> This has been raised several times here already. I would suggest to search the
> mailing list archive to learn why.
>
> Cheers,
>
> G.
>