Login  Register

Re: Brightness and contrast adjustment of single slice in stack

Posted by Wayne Rasband on Apr 01, 2009; 1:49pm
URL: http://imagej.273.s1.nabble.com/Brightness-and-contrast-adjustment-of-single-slice-in-stack-tp3693132p3693133.html

On Apr 1, 2009, at 6:55 AM, Stan Bindon wrote:

> I have stacks with 16-bit images, all of which I would like to adjust
> brightness and contrast while keeping them in the stack for  
> convenience.
> After searching the list, the solution posted in 2001 was to hold  
> down alt
> while turning on animation.
>
> Is there an easier/more straightforward way to do this?

Use the Image>Hyperstacks>Stack to Hyperstack command. In the dialog  
box, set "Channels:" to the number of images in the stack, "Slices:"  
to 1, "Frames:" to 1 and "Display Mode:" to "Grayscale".

-wayne