Re: generate ROIs around X,Y co-ordinate list

Posted by dpoburko on Mar 25, 2009; 8:18pm
URL: http://imagej.273.s1.nabble.com/Re-generate-ROIs-around-X-Y-co-ordinate-list-tp3693172.html

Hi All,

  I am trying to automate segmentation of small fluorescence events. In
the process, I was planing on using the Analyze particles to help with
this job. So far so good. But I am wondering how I might go about using
the centroid X.Y position from the particle analysis to then go back and
create ROIs of a predetermined size around the list of centroid
co-ordinates. In theory this shouldn't be hard, but I'm at a loss for
how to code it (i.e. gather the XY list from the results table and hand
it to the ROI manager or generate regions).

Thanks,
Damon

--

Damon Poburko, PhD
Postdoctoral Research Fellow
Stanford University School of Medicine
Dept. of Molecular & Cellular Physiology
279 Campus Dr., Beckman B103, Stanford, CA 94305
Ph: 650 725 7564, fax: [phone]