Macro/plugin to align channels ?

Posted by lechristophe on Mar 19, 2009; 10:05am
URL: http://imagej.273.s1.nabble.com/Macro-plugin-to-align-channels-tp3693202.html

Dear all,

On our confocal, we have objectives that are not well corrected for lateral
chromatic aberrations (see here :
http://micro.magnet.fsu.edu/primer/anatomy/aberrations.html). That means
that an image taken in three channels (with green, red and far-red emission
wavelength) will have a mismatch that has a radial variation (the green
image apperas "more zoomed" than the red which is "more zoomed" than the
far-red).
An image of three-color beads is worth a thousand words :
http://ftp.espci.fr/incoming/christo/Aberration.png

So I need to correct by changing each channel's image size (dilating far-red
and red images to make them fit with the green channel, which is the most
used channel and the reference). I don't need to have an automatic
registration, as the channels can have very different images (if not used to
image dumb beads), and the shift is constant for an objective (I set the
correction once for all images made with this objective).

So the question is : is there a macro/plugin that does that (correcting
radial shifts by changing R,G,B channels size by a fied amount) or do I have
to do it by myself ?

Thanks for your informations !

--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
[hidden email]