Re: Macro/plugin to align channels ?

Posted by lechristophe on Mar 19, 2009; 10:08am
URL: http://imagej.273.s1.nabble.com/Macro-plugin-to-align-channels-tp3693202p3693203.html

I forgot to specify that the linked image is a cropped, resized verion of
the original image (approximatively the upper left quarter of it). In the
full original image, the shift is realy radial with zero-shift at the center
and more shift as you get further from the center of the image.

On Thu, Mar 19, 2009 at 11:05, Christophe Leterrier <
[hidden email]> wrote:

> Dear all,
>
> On our confocal, we have objectives that are not well corrected for lateral
> chromatic aberrations (see here :
> http://micro.magnet.fsu.edu/primer/anatomy/aberrations.html). That means
> that an image taken in three channels (with green, red and far-red emission
> wavelength) will have a mismatch that has a radial variation (the green
> image apperas "more zoomed" than the red which is "more zoomed" than the
> far-red).
> An image of three-color beads is worth a thousand words :
> http://ftp.espci.fr/incoming/christo/Aberration.png
>
> So I need to correct by changing each channel's image size (dilating
> far-red and red images to make them fit with the green channel, which is the
> most used channel and the reference). I don't need to have an automatic
> registration, as the channels can have very different images (if not used to
> image dumb beads), and the shift is constant for an objective (I set the
> correction once for all images made with this objective).
>
> So the question is : is there a macro/plugin that does that (correcting
> radial shifts by changing R,G,B channels size by a fied amount) or do I have
> to do it by myself ?
>
> Thanks for your informations !
>
> --
> Christophe Leterrier
> Postdoc
> INSERM UMR641 // Ionic channels Lab
> IFR Jean Roche, Mediterranée University
> Marseille, France
> [hidden email]
>
>