http://imagej.273.s1.nabble.com/Macro-plugin-to-align-channels-tp3693202p3693205.html
It registers with bUnwarpJ all images in a stack to the first one. If
work. Just take into account the bUnwarpJ parameters and the fact that
> As your abberation shifts are not constant over the image you need a
> map of the distortion. If you can locate a version of UnwarpJ called
> StackUnwarpJ, you can use one channel as the reference (via copy) and
> align the RGB stack slices to perfection. Using the option to save the
> templates, you should be able to apply the same bead-defined
> corrections to other images. I no longer recall where I found the
> stack version, but it was likely via request from Carlos Sorzano.
> bUnwarpJ in 'mono' mode might do this the hard way (one channel at
> a time).
> cheers,
>
>
> Christophe Leterrier wrote:
>> I forgot to specify that the linked image is a cropped, resized
>> verion of
>> the original image (approximatively the upper left quarter of it). In
>> the
>> full original image, the shift is realy radial with zero-shift at the
>> center
>> and more shift as you get further from the center of the image.
>>
>> On Thu, Mar 19, 2009 at 11:05, Christophe Leterrier <
>>
[hidden email]> wrote:
>>
>>
>>> Dear all,
>>>
>>> On our confocal, we have objectives that are not well corrected for
>>> lateral
>>> chromatic aberrations (see here :
>>>
http://micro.magnet.fsu.edu/primer/anatomy/aberrations.html). That
>>> means
>>> that an image taken in three channels (with green, red and far-red
>>> emission
>>> wavelength) will have a mismatch that has a radial variation (the green
>>> image apperas "more zoomed" than the red which is "more zoomed" than
>>> the
>>> far-red).
>>> An image of three-color beads is worth a thousand words :
>>>
http://ftp.espci.fr/incoming/christo/Aberration.png>>>
>>> So I need to correct by changing each channel's image size (dilating
>>> far-red and red images to make them fit with the green channel,
>>> which is the
>>> most used channel and the reference). I don't need to have an automatic
>>> registration, as the channels can have very different images (if not
>>> used to
>>> image dumb beads), and the shift is constant for an objective (I set
>>> the
>>> correction once for all images made with this objective).
>>>
>>> So the question is : is there a macro/plugin that does that (correcting
>>> radial shifts by changing R,G,B channels size by a fied amount) or
>>> do I have
>>> to do it by myself ?
>>>
>>> Thanks for your informations !
>>>
>>> --
>>> Christophe Leterrier
>>> Postdoc
>>> INSERM UMR641 // Ionic channels Lab
>>> IFR Jean Roche, Mediterranée University
>>> Marseille, France
>>>
[hidden email]
>>>
>>>
>>>
>
Ctra. de Colmenar Viejo, Km. 15