measuring distances between circles

Posted by dfroelich on
URL: http://imagej.273.s1.nabble.com/measuring-distances-between-circles-tp3693283.html

I would like to automate this task.  I am trying to measure the minimum distances between neighboring colonies on agar plates.  
So far: {macro:
threshold - to convert the images to a binary,
invert - white colonies on a black background,
distance map - intensity values (0-255) correspond to neighboring colony distance}
Line tool - draw lines (by hand) between neighboring colonies, then measure/record minimum intensity values.

Is there a way to replace that last step so that imageJ draws lines between adjoining colony's centroids and records their minimum intensities?
Thank you
Daniel Froelich