Re: cell by cell local thickness

Posted by David Webster on Mar 14, 2009; 4:33am
URL: http://imagej.273.s1.nabble.com/cell-by-cell-local-thickness-tp3693294p3693295.html

Eric,

I'm assuming that you want to get to each cell's image and then run your
process on that cell alone. A way to do this 1)  is to thresholded a
multicell image to get an image of cell silhouettes, 2) use Landini's
BinaryLabel8 plugin (see plugins documentatiuon) to assign a unique label to
each cell silhouette, 3) use setThreshold() and run("Convert to Mask") to
pull out each cell's silhouette in turn, and 4) run your process on each of
these silhouettes.

Does this help, or am I missing the point?

David Webster
On Fri, Mar 13, 2009 at 6:39 AM, Eric BADEL <[hidden email]>wrote:

> Hi all,
> I need to evaluate the cell wall thickness in a cellular material. I
> already
> use "distance map" and other very useful "Local thickness" plugin and I get
> the map of local thickness.
> Now I need to split the information and to evaluate the local thickness of
> the wall of each cell. This mean I need to get all the values on the
> skeleton that is linked to one cell (and do it for each cell).
> I had the idea to use the skeleton image, to dilate the cell then make the
> difference with the initial image in order to get the values on the
> boundaries of the cell. But I do not know how to operate cell by cell.
> Any other idea ?
> Eric
>