Re: cell by cell local thickness

Posted by Gabriel Landini on Mar 16, 2009; 9:52am
URL: http://imagej.273.s1.nabble.com/cell-by-cell-local-thickness-tp3693294p3693297.html

On Monday 16 March 2009 08:50:00 Eric Badel wrote:
> I generated an image that represents only the boundaries of the cell.
> The grey level values on this boundary represent the corresponding local
> thickness. Nox, I need to get these values, cell by cell. That means
> that the boundary line should be one ROI. Nevertheless, if I try to get
> values of this ROI, ImageJ thinks that the ROI is the surface of the shape.
> How, could I define only the boundary as an ROI (and get all the
> corresponding grey level value) ?


What results do you want from those boundaries?

You could use the particles8 plugin on the binary boundaries images and
redirect to the distance transform image.
The mean, mode, max, min, sd, etc of those distance transform pixels
corresponding to the binary image will be collected in the Results window, one
row per cell.

I hope this helps.
G.