Re: removing out-of-focus objects

Posted by Robert Dougherty on Feb 02, 2009; 9:51pm
URL: http://imagej.273.s1.nabble.com/AVI-Virtual-Stack-1GB-boundery-tp3693422p3693433.html

Frank,

My favorite technique is to apply a small blur and subtract the  
blurred image from the original.  Areas that show a big change are  
likely to be in focus.  (Or maybe not, depending on the details of  
the optics.)

Bob

On Feb 2, 2009, at 11:42 AM, Franklin Shaffer wrote:

> I am taking high speed videos of 3D object (particle) flow fields  
> with a 2D high speed camera.  I have the classic problem in that  
> particles are in-focus in a plane and particles outside the plane  
> are out-of-focus.
>
> I am testing FFT bandpass and other filters to remove the out-of-
> focus particles.
>
> If anyone has experience with filters or ImageJ plugins that work  
> will for removing out-of-focus objects, please advise.
>
> Thank you,
> Frank Shaffer
>
> DOE National Energy Technology Laboratory

Robert Dougherty, Ph.D.
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