Posted by
Stephan Preibisch on
Feb 02, 2009; 8:08pm
URL: http://imagej.273.s1.nabble.com/AVI-Virtual-Stack-1GB-boundery-tp3693422p3693444.html
Dear Frank,
I wrote an Autofocus plugin for our brightfield microscope using
FFT-bandpass filter which did a good job. It performs better than comparing
the standard deviation of the whole image (concerning quality and time). But
I am so not sure it does a good job for finding particles which are out of
plane.
As you have the advantage that you do not have to do it real time (I think),
you could try Mr. Saalfelds feature detection plugin
(
http://fly.mpi-cbg.de/~saalfeld/javasift.html). It detectes features of
different sizes in the image. You could try to analyze the occurrence and
reason from the detected object sizes whether they are in focus or not.
All the best,
Stephan
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
Franklin Shaffer
Sent: Monday, February 02, 2009 8:42 PM
To:
[hidden email]
Subject: removing out-of-focus objects
I am taking high speed videos of 3D object (particle) flow fields with a 2D
high speed camera. I have the classic problem in that particles are
in-focus in a plane and particles outside the plane are out-of-focus.
I am testing FFT bandpass and other filters to remove the out-of-focus
particles.
If anyone has experience with filters or ImageJ plugins that work will for
removing out-of-focus objects, please advise.
Thank you,
Frank Shaffer
DOE National Energy Technology Laboratory