Re: Roi Enlarge (without merging) / ROI merging / Edges to ROI conversion

Posted by sazzad on
URL: http://imagej.273.s1.nabble.com/Roi-Enlarge-without-merging-ROI-merging-Edges-to-ROI-conversion-tp3693456p3693460.html

Gabriel Landini wrote

On Wednesday 26 November 2008 17:51:02 Ghislain Bonamy wrote:
> Thanks for the prompt answer. I was thinking that reducing the particle
> size was easy enough via a mask transform and an erosion. It is possible
> that particles may become separated, but there isn't in my opinion a much
> better way to do it (unless one can directly resize an ROI).

Perhaps something like some iterations of thinning, would do it.

> For the seeded watershed, I look on the pugin page and found the two
> plugins you are reffering to, but they do not seem to be seeded watershed
> algorithm. Perhaps, I am missing something.

Like Michael said, you could provide a new image which has only your seeds and
apply the watershed to that.
Another possibility is to skeletonise the background (skiz, or influence
zones).

> I think your solution of redilate-no-merge is a great solution. Am I
> correct to believe that the plugin you are reffering to is the one you
> created in your morphology set?

Yes it is, but be aware that for particles that are separated by exactly 2
pixels thick space, one cannot dilate both objects. Since the algorithm
dilates all particles independently in a sequence, one will be dilated and the
other won't.

Cheers
G

I have got a question.

If there is a neucleaus in a cell do you think watershed algorithm will be able to solve the problem?

If yes then is there anything that we can do?

How can we try in that case?

cheers