Login  Register

Re: FFT on a stack ( automatization)

Posted by Joel Sheffield on Mar 02, 2009; 12:51pm
URL: http://imagej.273.s1.nabble.com/FFT-on-a-stack-automatization-tp3693511p3693513.html

You might also try the FFT Bandpass filter.  

Joel


Date sent:       Mon, 2 Mar 2009 10:06:42 +0100
Send reply to:   ImageJ Interest Group <[hidden email]>
From:           Michael Schmid <[hidden email]>
Subject:         Re: FFT on a stack ( automatization)
To:             [hidden email]

> Hi Guenther,
>
> Process>FFT>Custom filter works on stacks.
>
> Take our FFT, make a mask from it that is 255 where wou want to pass  
> the frequencies and 0 where you want to block it (essentially the  
> same as you would do for normal filtering, but you have to change the  
> values both inside *and* outside of your roi). Then smooth it  
> slightly (3 times 'smooth' or Gaussian Blur with a small radius to  
> aviod ringing artifacts).
>
> In contrast to normal 'Inverse FFT', for 'Custom Filter' you have to  
> take care about creating a symmetric filter, it won't be made  
> symmetric with respect to the origin.
>
> Michael
> ________________________________________________________________
>
> On 2 Mar 2009, at 07:45, Guenter Giese wrote:
>
> > Dear list members,
> >
> > I have stacks of images with darkened regions (shadow-like stripes)  
> > running
> > through (two main directions with angles only some degrees to  
> > orthogonal
> > directions). On a single image, I run FFT, edit the power spectrum  
> > (clearing
> > of a polygonal ROI), and then do inverse FFT.
> >
> > Is it possible to do automated FFT/ROI clearing/inverse FFT on a  
> > whole stack
> > using a polygonal (predefined?) ROI for editing the power spectrum?  
> > Perhaps
> > as a macro?
> >
> > Best,
> >
> > Guenter
> >
> > ------------------------------------------
> > Dr. Guenter Giese
> > Light Microscopy Facility Manager
> > Dept. of Biomedical Optics
> > MPI fuer Medizinische Forschung Jahnstr. 29
> > D-69120 Heidelberg, Germany
> > Phone (+49) 6221-486-360 (Fax: -325)
> > e-mail: [hidden email]



Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs