Re: Re; Nuclear volume, ploidy and Stain stochiometry

Posted by Alton D Floyd on Feb 18, 2009; 6:48pm
URL: http://imagej.273.s1.nabble.com/Re-Nuclear-volume-ploidy-and-Stain-stochiometry-tp3693629p3693630.html

For autoradiographic grain counting, the type of emulsion, developer
used, and method of development all influences the grain size and
morphology.  When this technique was widely used, in the early 1960's,
most investigators used developers that produced tiny, round grains.  As
in any other type of quantitative measurement, the specimen preparation
and grain development must be highly controlled.  

When I was actively involved in this type of work, we found that we had
to create new calibrations each time we used a new batch of emulsion.
The calibration was created by making a series of slides, coating with
emulsion, and exposing for increasing lengths of time.  Then we took
reflectance measurements, and compared these to actual grain counts in
the measured areas.  With such a calibration, we found excellent
correlation between actual counts and reflectance measurements.
Obviously, the measurements were much faster, and permitted studies such
as localization of radioactive tagged materials in serial sections of
organs, such as rodent brains.

I don't remember all of the citations to these type of studies.  I will
check my old citation card file and see if I can come up with specific
citations that will give you a starting point for a citation search.

Al Floyd
23126 South Shore Drive
Edwardsburg, MI 49112
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