Posted by
Glen MacDonald-2 on
Feb 18, 2009; 12:01am
URL: http://imagej.273.s1.nabble.com/Re-IMAGEJ-Digest-14-Feb-2009-to-15-Feb-2009-2009-45-tp3693650p3693653.html
Hello,
A colleague is recording the swimming motions of zebrafish larvae in
response to water flow, using a macro lens on a CCD camera. We need
to determine the angle of orientation relative to the x-axis. The
larvae are thresholded in a timelapse stack and Analyze Particles
provides the information, with parameters set for exclude edges,
minimum size and circularity below .26 (the larvae resemble small
nails at this magnification with a tapered body and a bulge at the
head).
Analyze Particles correctly identifies about 25 larvae in each frame
of the timelapse. But, 3-5 larvae are not measured in each frame.
The display of the best fit ellipses and numbering the thresholded
larvae by result line identifies the missed larvae. Individual
measurement of the missed larvae indicates they meet all criteria for
particle analysis. In some instances, a larva may not have changed
position for several frames, yet will have been measured in some
frames, but not in others.
Thanks,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
[hidden email]
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The box said "Requires WindowsXP or better", so I bought a Macintosh.
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