Posted by
Hugo A. M. Torres on
Feb 13, 2009; 12:13pm
URL: http://imagej.273.s1.nabble.com/Macros-to-read-and-write-Complex-Fourier-Transform-tp3693680p3693684.html
No Joris, I understand what you are saying, but the problem is the first
ROI (used to measure the nucleus I am interested in) has a triangular
shape, which will not fit in the background area unless I make it into a
square of the same area as the triangle. Now, this should be very
straightforward: just draw a square with sides whose lenght is the
square root of the triangle area. It doesn't work if I use a calculator,
possibly because of rounding issues, I don't know.
I always get a smaller area in um^2 if I do this by hand. So I was
wandering if it is possible to specify a square ROI by area value.
Any ideas?
Tganks
On Fri, 2009-02-13 at 11:08 +0100, joris meys wrote:
> The measurements should be fairly simple with the ROI manager. Just click on
> the roi you specified earlier, and drag it to where you want to measure.
> First click "Add", because otherwise the ROI manager will measure at the
> previous position. That should give you the results you want.
>
> Kind regards
> Joris
>
>
> On Fri, Feb 13, 2009 at 4:36 AM, Hugo A. M. Torres <
[hidden email]>wrote:
>
> > Hello listmates,
> >
> > I am trying to perform densitometry measurements according to this
> > method:
> >
> > "The nucleus was encircled by free-hand drawing of a border around the
> > outermost mRNA-expressing cells in the nucleus. The mean density and the
> > area of the selection were measured. The mean density of the background
> > was measured from a rectangular area of corresponding size in the
> > immediate vicinity of the nucleus. The mean density of the background
> > was subtracted from the mean density of the nucleus and..."
> >
> > Now, the problem is I can't specify a ROI of the same area of the place
> > I measured previously. I tried taking the square root of the area (given
> > in um^2) and specifying a square ROI with sides of this length but the
> > resulting area measured is always smaller!
> >
> > Is it possible to specify a square ROI by inputing an area value in
> > square micrometers?
> >
> > Thanks
> > --
> > Hugo Arruda de Moura Torres
> > ==================================
> > Departamento de Biofísica
> > Universidade Federal de São Paulo
> > Rua Botucatu 862 7o. andar
> >
--
Hugo Arruda de Moura Torres
==================================
Departamento de Biofísica
Universidade Federal de São Paulo
Rua Botucatu 862 7o. andar
CEP 04023-062 Vila Clementino
São Paulo - SP - Brasil
Tel:+55 (11) 5576 4530 r.220
Fax: 55 11 5571 5780