Posted by
"Janne Hyötylä" on
Feb 09, 2009; 2:26pm
URL: http://imagej.273.s1.nabble.com/Tudor-DICOM-compressed-images-tp3693776p3693779.html
On Mon, 09 Feb 2009 14:43:44 +0100, Hugo A. M. Torres
<
[hidden email]> wrote:
> Hello dear colleagues,
>
> ImageJ seems to be displaying some files incorrectly here. Please check
> this link:
http://imagebin.ca/view/6z_mf8hh.htmler>
> There I have the imageJ and another application (nautilus) displaying
> the same TIF file. The imageJ display seems more grainy and darker.
> The interesting thing is that it does not happen to every file! Specimen
> photos are displayed as expected.
Hello,
ImageJ changes the value for white and and black according to the maximal
and minimal intensities in an image. This only affects how the image is
displayed, not the raw data itself. You can check this yourself by going
to Image > Adjust > Brightness/Contrast. You will see that in your case
the histogram does not go from 0 to 255 (or 0-65535 in 16bit) but a subset
of it. But when you change the sliders the raw intensity of each pixel
does not change. Any operation (subtraction etc.) will act on the raw
values so this will not be a problem.
Nautilus on the other hand seems to fix black = 0 and white = 255 (or
65535).
Best regards,
Janne
--
Janne Hyötylä
Biozentrum / Swiss Nanoscience Institute
University of Basel
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