Re: anomalous (?) display

Posted by Hugo A. M. Torres on Feb 09, 2009; 9:25pm
URL: http://imagej.273.s1.nabble.com/Tudor-DICOM-compressed-images-tp3693776p3693784.html

The Wayne Rasband's "Shading Corrector" plugin does exactly what I need
in a very straightforward way! All I have to do is rename my light path
image to "blank-field" without  the  file extension, open both images
and click on its menu entry.

Hope this helps others


On Mon, 2009-02-09 at 15:20 -0500, Gabriel Lapointe wrote:

> In the Brightness and contrast dialog ther is a "set" button where you
> can enter the values manually.
>
> Hope this help,
>
> Gabriel
>
> Hugo A. M. Torres wrote:
> > Thanks Gabriel,
> >
> > I will try to do the operation before the conversion, now if I had to do
> > it later, how would I ensure the contrast is the same? Should I worry
> > only about the slope and position of the line displayed over the
> > histogram  in the b/c dialogue box? Sorry if this is basic, I am new at
> > this.
> >
> > Hugo
> >
> > On Mon, 2009-02-09 at 12:55 -0500, Gabriel Lapointe wrote:
> >
> >  
> >> Hello Hugo,
> >> When you convert a 16 bit image to 8bit ImageJ take use the minimum and
> >> maximum displayed as the limits for the scaling down. So you either have
> >> to adjust the brightness and contrast before the conversion (make sure
> >> it is the same for all sample) or avoid the conversion and substract the
> >> 16 bit image directly. As long as both image are the same depth (8 or 16
> >> bit) it should work.
> >>
> >> Gabriel
> >>
> >>    
> >
> >  

--
Hugo Arruda de Moura Torres
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