Re: subtracting average

Posted by David Hovis on Jan 30, 2009; 8:19pm
URL: http://imagej.273.s1.nabble.com/subtracting-average-tp3693882p3693883.html

Sounds to me like you want to do Image->Stacks -> Z-project (Average  
Intensity).

Then you can use Process-> Image Calculator... (Image1:Original_Stack,  
Operation: Subtract, Image2: AVG_Original_Stack) to subtract the  
average frame from each frame in the stack.

You'll probably want to use a 32-bit result, as otherwise any value  
less than the average will become zero.

--David


On Jan 30, 2009, at 5:54 AM, chris elliott wrote:

> Hi, I have an avi which i can read withimageJ, but I wanted to  
> calculate the average intensity at each corresponding pixel, and  
> then subtract this from each frame. Is there an existing plugin/
> macro etc to do this?
>
> thanks
> chris