Re: Overlay with pseudocolor image

Posted by dscho on Jan 16, 2009; 1:01pm
URL: http://imagej.273.s1.nabble.com/Overlay-with-pseudocolor-image-tp3694042p3694043.html

Hi,

On Fri, 16 Jan 2009, Sofia Domingues wrote:

> I would like to overlay two images in ImageJ and failed up to now. One
> image is a grayscale image from the brightfield acquisition. However,
> the other one is a pseudocolor image, derived from a ratiometric CFP/YFP
> FRET image acquisition. Neither in Metamorph nor in ImageJ I was able to
> overlay these two images up to now as either the pseudocolor image can
> not be selected at all or the information of the pseudocolor gets lost
> as the image got flattened to a single color ...

If I were to overlay a gray image with a pseudocolor image, I would
convert both to RGB color and then use Process>Image Calculator to add
them.

But I guess I did not understand what you mean by "or the information of
the pseudocolor gets lost as the image got flattened to a single color".  
Maybe you want to be a little more verbose about it, so I have a chance to
understand what you need?

Ciao,
Dscho