Re: "stacks"

Posted by David Hovis on Jan 12, 2009; 5:40pm
URL: http://imagej.273.s1.nabble.com/stacks-tp3694045p3694046.html

I think "stack" generally refers to a series of related images, in  
some sort of order.  Usually they have the same X-Y field and vary in  
Z, Time, Wavelength, or something else.  ImageJ is fairly agnostic  
about what you put in a stack, but if the data is confocal, then the  
"stack" probably varies in Z, Time or Wavelength.

Certainly I use ImageJ to analyze "stacks" of confocal data.  Usually  
Z-stacks in my case for topography measurements.

--David

On Jan 12, 2009, at 11:20 AM, Robert J. Palmer Jr. wrote:

> Is the term "stack" in Image J translatable to that as used in  
> confocal or digital deconvolution microscopy?  I am struggling with  
> an assertion that processing of "stacks" in Image J was used to  
> analyze height of a feature.  Perhaps in this case the phrase  
> "collecting stacks of images" refers to a group of images collected  
> in x-y?  This is my current understanding of the term "stack" as  
> used in Image J - a series of related images that needn't  
> necessarily represent any one particular field-of-view.
> --
> Robert J. Palmer Jr., Ph.D.
> Natl Inst Dental Craniofacial Res - Natl Insts Health
> Oral Infection and Immunity Branch
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