Re: Grienkiwicz equation: confused

Posted by karo03 on Dec 23, 2008; 12:46pm
URL: http://imagej.273.s1.nabble.com/Grienkiwicz-equation-confused-tp3694185p3694186.html

I am not involved with Ca concentration. However, under http://www.jbc.org/cgi/reprint/260/6/3440 
  page 3447 your problem is possibly outlined.

Regards
Karsten

Am 22.12.2008 um 07:28 schrieb RajGourob Ghosh:

> Dear All,
>
>              I have to calculate the calcium conc. using the  
> Grienkiwicz
> equation. I am not able to understand the difference between the  
> calculation
> of Beta and Rmin, Rmax, as from the reports I have read I can see  
> both are
> ratios but then is it that Beta is calculated only on slide without  
> the
> sample while Rmin and Rmax are deciphered from slides loaded with  
> sample. I
> am confused in this regard . Someone please help.
>
> [Ca ]i = KD x ß x (R -Rmin) / (Rmax- R)
>
> I have no idea of the difference between
> ß = fluorescence signal at 351nm at 0
> μmol.l-1 Ca2+ / fluorescence signal at 364 nm at 39.8 μmol.l-1 Ca2+.
> Rmin = ratio at 0 mmol.l-1 Ca2+.
> Rmax = ratio at 39.8 μmol.l-1 Ca2+.