http://imagej.273.s1.nabble.com/Grienkiwicz-equation-confused-tp3694185p3694187.html
> I am not involved with Ca concentration. However, under
>
http://www.jbc.org/cgi/reprint/260/6/3440 page 3447 your problem is
> possibly outlined.
>
> Regards
> Karsten
>
> Am 22.12.2008 um 07:28 schrieb RajGourob Ghosh:
>
>
> Dear All,
>>
>> I have to calculate the calcium conc. using the Grienkiwicz
>> equation. I am not able to understand the difference between the
>> calculation
>> of Beta and Rmin, Rmax, as from the reports I have read I can see both are
>> ratios but then is it that Beta is calculated only on slide without the
>> sample while Rmin and Rmax are deciphered from slides loaded with sample.
>> I
>> am confused in this regard . Someone please help.
>>
>> [Ca ]i = KD x ß x (R -Rmin) / (Rmax- R)
>>
>> I have no idea of the difference between
>> ß = fluorescence signal at 351nm at 0
>> μmol.l-1 Ca2+ / fluorescence signal at 364 nm at 39.8 μmol.l-1 Ca2+.
>> Rmin = ratio at 0 mmol.l-1 Ca2+.
>> Rmax = ratio at 39.8 μmol.l-1 Ca2+.
>>
>