Re: Grienkiwicz equation: confused

Posted by swaraj basu on Dec 23, 2008; 4:21am
URL: http://imagej.273.s1.nabble.com/Grienkiwicz-equation-confused-tp3694185p3694188.html

On Mon, 22 Dec 2008 RajGourob Ghosh wrote :
>Dear All,
>
>
 


On Mon, 22 Dec 2008 RajGourob Ghosh wrote :

>Dear All,
>
>               I have to calculate the calcium conc. using the Grienkiwicz
>equation. I am not able to understand the difference between the calculation
>of Beta and Rmin, Rmax, as from the reports I have read I can see both are
>ratios but then is it that Beta is calculated only on slide without the
>sample while Rmin and Rmax are deciphered from slides loaded with sample. I
>am confused in this regard . Someone please help.
>
>[Ca ]i = KD x ©¬ x (R -Rmin) / (Rmax- R)
>
>  I have no idea of the difference between
> ʩ = fluorescence signal at 351nm at 0
>  ¥ìmol.l-1 Ca2+ / fluorescence signal at 364 nm at 39.8 ¥ìmol.l-1 Ca2+.
>  Rmin = ratio at 0 mmol.l-1 Ca2+.
>  Rmax = ratio at 39.8 ¥ìmol.l-1 Ca2+.

Hello Rajgourob,
                Even I am perplexed by the same equation, I have gone through a few papers, being a novice in this area I have a faint idea how should the slides be prepared to get Rmin Rmax and Beta. With no one to help in my institute I have just hung up on the situation for the time being.
                 Swaraj