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Cross section area of muscle, less the fat

Posted by casab on Dec 13, 2008; 7:22pm
URL: http://imagej.273.s1.nabble.com/Cross-section-area-of-muscle-less-the-fat-tp3694251.html

On MRI images, I am trying to measure the cross sectional area of muscle after subtracting out the fat component. I figured out one way to do it with ImageJ, but it is very labor intensive. Basically, I draw a region of interest, make a histogram of the pixel intensities, cut/paste the histogram list into excel, manually choose a pixel intensity cut-off between fat and muscle, count pixels on "muscle side" of cut-off, and then convert the number of pixels into an area measurement. Does anyone know of a faster or automated way to do this? Perhaps there is a different software program that is free or inexpensive that can do this? The attached image is an example of what I would like to do, performed with a Korean program called Rapidia. Unfortunately, that program is quite expensive. Any ideas? Thanks