Hi, Ben
thanks for your answer
I have used the Multi Measure in the ROI manager.
If I understand you correctly this should give me the average - intensity/area and this is already normalized data
Shilo
On Dec 8, 2008, at 7:56 AM, shilo2 wrote:
> Dear list members
> Maybe it's a stupid question but because I'm a beginner I must ask
> In a mistake I took some pictures (confocal microscope) in low
> resolution
> (256*256). The majority are in more high resolution (512*512). Now,
> I would
> like to compare intensity's.
> Does the resolution should affect the intensity means? If the
> answer is yes
> , is there a way to correct it?
>
Hi,
That doesn't seem like a stupid question to me. I assume you mean
greyscale intensity - in which case your best way to make the
comparison is to "normalize" the integrated density by area. This
gives you the mean grey value which is one of ImageJ's measurement
selections (see http://rsb.info.nih.gov/ij/docs/menus/analyze.html#set).
normalized grayness = mean grey value = (integrated density) / area
Once normalized, you can reasonably compare across different
resolutions.*
Hope that helps.
Cheers,
Ben
* There are other issues involved, including how the pixel binning
was done between the low and high resolution. If you need to dig
into that you'll have to consult your camera documentation and an
image processing reference. This one (http://www.imagingbook.com/) is
a goodie.
This email is a reply to your post @ http://n2.nabble.com/resolution-affect-intensity--tp1628924p1629209.html
You can reply by email or by visting the link above.
| Free forum by Nabble | Disable Popup Ads | Edit this page |