Re: resolution affect intensity?

Posted by Michael Doube on Dec 08, 2008; 3:15pm
URL: http://imagej.273.s1.nabble.com/resolution-affect-intensity-tp3694298p3694302.html

I agree with Gabriel; yes the intensities will be different. Unless you
know everything about your confocal you will not be able to compare
brightnesses across images of different resolutions.  It is hard enough
when you do your best to keep everything exactly the same...  For
example, the Leica SP5 automagically adjusts for resolution effects on
brightness but the earlier Leica SP2 does not.  So you must know what
the automagic is before trying to do any correction to your broken
images.  Much better is just to curse your mistake and do the imaging again.

Mike

Gabriel Landini wrote:

> On Monday 08 December 2008 14:40:54 shilo2 wrote:
>> I have used the Multi Measure in the ROI manager.
>>
>> If I understand you correctly this should give me the average -
>> intensity/area and this is already normalized data
>
> You most likely changed various settings in the microscope to get a reasonable
> image in both cases, so image intensity is unlikely to be directly comparable
> across magnifications.
>
> Cheers,
> G.