Re: resolution affect intensity?

Posted by shilo2 on Dec 08, 2008; 3:25pm
URL: http://imagej.273.s1.nabble.com/resolution-affect-intensity-tp3694298p3694303.html

I udersatnd, no shortcuts
 Thank you all

Shilo



On Mon, Dec 8, 2008 at 5:15 PM, Michael Doube <[hidden email]>wrote:

> I agree with Gabriel; yes the intensities will be different. Unless you
> know everything about your confocal you will not be able to compare
> brightnesses across images of different resolutions.  It is hard enough when
> you do your best to keep everything exactly the same...  For example, the
> Leica SP5 automagically adjusts for resolution effects on brightness but the
> earlier Leica SP2 does not.  So you must know what the automagic is before
> trying to do any correction to your broken images.  Much better is just to
> curse your mistake and do the imaging again.
>
> Mike
>
>
> Gabriel Landini wrote:
>
>> On Monday 08 December 2008 14:40:54 shilo2 wrote:
>>
>>> I have used the Multi Measure in the ROI manager.
>>>
>>> If I understand you correctly this should give me the average -
>>> intensity/area and this is already normalized data
>>>
>>
>> You most likely changed various settings in the microscope to get a
>> reasonable image in both cases, so image intensity is unlikely to be
>> directly comparable across magnifications.
>>
>> Cheers,
>> G.
>>
>