Re: resolution affect intensity?

Posted by karo03 on Dec 08, 2008; 2:37pm
URL: http://imagej.273.s1.nabble.com/resolution-affect-intensity-tp3694298p3694304.html

Typically cl(sm) parameters are changed, changing the resolution or  
optics. I would think mean intensities of regions are not directly  
comparable. At least a different gain factor is to be expected. I  
would calculate that gain factor by measuring similar objects which  
should have the same (mean) intensity. The additive offset might be  
zero for fluerescent images.
Regards
Karsten

Am 08.12.2008 um 13:56 schrieb shilo2:

> Dear list members
> Maybe it’s a stupid question but because I'm a beginner I must ask
> In a mistake I took some pictures (confocal microscope) in low  
> resolution
> (256*256). The majority are in more high resolution (512*512). Now,  
> I would
> like to compare intensity's.
> Does the resolution should affect the intensity means? If the answer  
> is yes
> , is there a way to correct it?
>
> Thank you
>
>
> --
> View this message in context: http://n2.nabble.com/resolution-affect-intensity--tp1628924p1628924.html
> Sent from the ImageJ mailing list archive at Nabble.com.