Re: resolution affect intensity?

Posted by David Hovis on Dec 08, 2008; 2:20pm
URL: http://imagej.273.s1.nabble.com/resolution-affect-intensity-tp3694298p3694305.html

It certainly can, depending on your microscope.  Changing your  
resolution can change your raster speed or your time/pixel.  Some  
instruments will attempt to auto-adjust the PMT voltages when you make  
a change.

The only way to find how big the effect might be would be to take  
sequential images at 256 and 512 resolutions (on a sample that won't  
photobleach during scans) and compare.

--David


On Dec 8, 2008, at 7:56 AM, shilo2 wrote:

> Dear list members
> Maybe it’s a stupid question but because I'm a beginner I must ask
> In a mistake I took some pictures (confocal microscope) in low  
> resolution
> (256*256). The majority are in more high resolution (512*512). Now,  
> I would
> like to compare intensity's.
> Does the resolution should affect the intensity means? If the answer  
> is yes
> , is there a way to correct it?
>
> Thank you
>
>
> --
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