Biased/stretched LUTs in ImageJ

Posted by John Oreopoulos on Nov 21, 2008; 5:54pm
URL: http://imagej.273.s1.nabble.com/doesn-t-recognize-ratio-command-tp3694424p3694429.html

Hi,

I have a calculated image formed by the ratiometic combination of two  
other images. I want to preserve the resulting pixel intensities in  
the calculated image
which is 32-bit. I have several calculated images like these and  
their pixel intensities vary widely. I'd like to display these images  
with a colored look up table
but be able to stretch LUT to bias the contrast to certain values for  
each image in a similar manner to what was done in Figure 3 and  
Figure 5 of the
following paper:

Parasassi, T., E. Gratton, W.M. Yu, P. Wilson, and M. Levi, 1997. Two-
photon fluorescence microscopy of Laurdan generalized polarization  
domains in model and natural membranes. Biophysical Journal 72:  
2413-2429.

When I take my calculated images and add a colored calibration bar  
for the LUT, I can adjust the brightness and contrast to get the  
desired effect, but the
pixel intensities no longer match the original calculated image. Is  
there a way to do this properly in ImageJ without altering my pixel  
intensities?

John Oreopoulos