Re: ratiometric images

Posted by shilo on Nov 20, 2008; 11:59am
URL: http://imagej.273.s1.nabble.com/ratiometric-images-tp3694452p3694453.html

Thank you all for your great help!!! it works.

Shilo

shilo wrote

Dear list
I'm sending the massage again because I wasn't subscribed.

I would like to produce ratiometric images from two stacks. The two pictures come from confocal microscope and each one of them is from different excitation wavelength.
I was trying to make it with the calculator plus tool (divide), but I got image with 0,1,2 values and not continues values.
In the next step I would like to have colors that represent the ratio values and scaling bar

Thanks for your help
Shilo