Re: ratiometric images

Posted by Michael Schmid on Nov 19, 2008; 6:18pm
URL: http://imagej.273.s1.nabble.com/ratiometric-images-tp3694452p3694455.html

Hi Shilo,

use Process>Image Calculator and check "Create New Window" and "32-
bit Result".
8-bit or 16-bit images can hold only integer numbers; "32-bit" is the  
floating point format.

For your other questions, see Image>Lookup Tables and  
Analyze>Tools>Calibration Bar (grayscale calibration) and  
Analyze>Tools>Scale Bar (spatial calibration).

Michael
________________________________________________________________

On 19 Nov 2008, at 18:35, shilo wrote:

> I would like to produce ratiometric images from two stacks. The two  
> pictures
> come from confocal microscope and each one of them is from different
> excitation wavelength.
> I was trying to make it with the calculator plus tool (divide), but  
> I got
> image with 0,1,2 values and not continues values.
> In the next step I would like to have colors that represent the  
> ratio values
> and scaling bar
>
> Thanks for your help
> Shilo