Images of axons

Posted by Va Paula Sicurello on Nov 18, 2008; 11:50pm
URL: http://imagej.273.s1.nabble.com/Images-of-axons-tp3694484.html

Hello Everybody,

I have a user here who needs to be able to distinguish between two different sizes of axons, those 50um and under and those 51um and over. I think she measures along the longest side because some are more oval or odd shaped than round.

The images are at  

http://www.vmrf.org/researchcenters/confocal/gallery.html 

The images of interest are on the bottom of the page with no description, they are .gif format.

Because these sections are stained with toluidine blue and saved as gray scale, Image Pro Plus is having a hard time distinguishing between the two sizes.  It sees the image as one big gray image with no distinct internal features.  We haven't tried ImageJ as yet.

I know the images are of poor quality, out of focus and bad resolution but this is what we have to work with.  I'm guessing that

If you have any suggestions as to how to make this counting process easier please let us know your suggestions.

Reply to either [hidden email] or to me.

Thank you for any help you may send us.

Paula  :-)

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397