Re: Pixel intensities at equidistant sample points along selection

Posted by Wayne Rasband on Nov 14, 2008; 3:13pm
URL: http://imagej.273.s1.nabble.com/Pixel-intensities-at-equidistant-sample-points-along-selection-tp3694489p3694490.html

On Nov 14, 2008, at 9:30 AM, Richard Mort wrote:

> Hi can anyone help me with this?
> I'd like to do a profile plot that returns pixel intensities at
> equidistant sample points along a selection and I'd like to be able to
> specify the distance.

The Edit>Selection>Fit Spline command, when called with a ""straighten"
option, converts a line selection to one with points spaced one pixel
apart. Here is an example:

   newImage("Test", "8-bit Black", 480, 480, 1);
   makeLine(77,81,186,97,246,165,248,236,180,310,88,292);
   getSelectionCoordinates(x,y);
   print("points: ", x.length);
   run("Measure");
   print("length: "+getResult("Length", nResults-1));
   run("Fit Spline", "straighten");
   getSelectionCoordinates(x,y);
   print("points: ", x.length);
   run("Measure");
   print("length: "+getResult("Length", nResults-1));

-wayne