Re: Filling the particles-bubbles by using threshold

Posted by Alexey on Nov 10, 2008; 10:59pm
URL: http://imagej.273.s1.nabble.com/Filling-the-particles-bubbles-by-using-threshold-tp3694547p3694551.html

First of all you should transform your image to binary using "Binary"->"Make
Binary" . In order to increase the performance of the main step you will
need to use a filter which closes the gaps in bubbles boundaries. For this
you can use "Binary"->"Close" as a first attempt and anisotropic diffusion
for the further improvement.
And the main step: "Binary"->"Fill Holes" algorithm. It will fill inner
parts automatically.

All the best,
Alexey

saule wrote

Hello, I'm currently analising bubble size change by using Image J. Aftersubstracting background and increasing contrast, I have tried to adjust thethreshold. However, I have to do a lot of work to clean, and erase all noiseand rubish around the bubbles. Is there's any way of doing an inverse picture or filling up the bubble inner part, so I could avoid cleaning step.


Sample of the picture 1_t000c3.jpg

Thank you very much,
Daiva