Re: 32bit colocalization plugin

Posted by Tony Collins-4 on Nov 03, 2008; 3:43pm
URL: http://imagej.273.s1.nabble.com/32bit-colocalization-plugin-tp3694593p3694594.html

Hi Colin,

May be tricky. The original algorithm steps through the data setting a threshold at "intensity/2" until it finds one where the Pearson's value is zero. With a 32-bit image this would require too many steps!

I'm also not sure 32-bit would give you much more information. Pearson's coefficient normalises the data anyway so you may want to scale it to 16-bits.

Hope this helps,

Tony

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Colin Rickman
Sent: November-03-08 6:17 AM
To: [hidden email]
Subject: 32bit colocalization plugin

Hi,

I currently use the colocalization threshold plugin to calculate the
linear regression between two channels. At present this plugin can only
use 8bit or 16bit unsigned byte images. Is it possible to change the
plugin to accept 32bit float images or is there another plugin out there
which can do this.

Hoe you can help

Colin

--
Dr Colin Rickman
Centre for Integrative Physiology
School of Biomedical Sciences
University of Edinburgh
Hugh Robson Building
George Square
Edinburgh
EH8 9XD

Tel: [phone]
Fax: [phone]


The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.