segmentation problen

Posted by Matthias Kirsch on Oct 31, 2008; 9:25am
URL: http://imagej.273.s1.nabble.com/segmentation-problen-tp3694606.html

Dear all,

I am trying to accurately segment cells from immunostainings. Identifying cells is in a first trial based on staining nuclei. As can be seen in the accompanying image, the "nucleus counter" plugin of ImageJ performs reasonably well with most nuclei, and even with some which are touching. Nevertheless, there are many where neither he plugin nor any other way I have tried, succeeds in doing this satisfactorliy for real life images. Now one would imagine, that there is enough information in the pair of nuclei slightly off-centre towards 11:00 hours in the sample image, to identify it as two, like center of mass based on their non-touching outlines. Howver, my knowledge of how to use this information is more than limited.

I would appreciate suggestions how to solve that problem. As the project advances, I certainly will stumble on more.

Thanks very much.

Matthias