Thresholding help

Posted by Justin Walker-3 on Oct 29, 2008; 6:56pm
URL: http://imagej.273.s1.nabble.com/Thresholding-help-tp3694620.html

I have a series of images of liquid droplets, which have very poor
contrast with the surrounding phase, due to the similarities in their
index of refraction.  I get enough contrast in my images to clearly see
the droplets, however, I can't pick them out with the threshold because
there is a slight gradient in the brightness of my lighting.  Link to a
sample image is below:

http://www.chejrw.com/forum/test.tif

Does anyone know of a way I can manipulate this image to be able to use
the 'measure particles' plugin to get the droplet sizes?  I can clearly
see the droplets, so surely there must be a way to make the computer see
them.

Thanks everybody

- Justin Walker