Re: high speed video with varying illumination

Posted by Frederic V. Hessman on
URL: http://imagej.273.s1.nabble.com/high-speed-video-with-varying-illumination-tp3694724p3694728.html

If the particles stay in the field of view or if the number of  
particles is large and hence approximately constant, then try  
subtracting the background (presumedly also varying due to scattering)  
and then normalize by the integrated brightness of the whole image.

Rick

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On 17 Oct 2008, at 12:21 am, Franklin Shaffer wrote:

> I'm taking high speed videos of particles at 10,000 frames per  
> second, but the light source I'm using for illumination is varying  
> in brightness at 60 hz.
> The result is that the brightness of each image is different, even  
> when the object being viewed is not changing.
>
> Anyone have any suggestions for image processing steps to correct  
> for the varying illumination?
>
> (I don't have the option of getting a different light source.)
>
> Thank you,
>
> Frank Shaffer
> Research Engineer
> USDOE National Energy Technology Laboratory
> Computational Science Division