Re: high speed video with varying illumination

Posted by Raymond Lillard on Oct 16, 2008; 11:21pm
URL: http://imagej.273.s1.nabble.com/high-speed-video-with-varying-illumination-tp3694724p3694730.html

Franklin,

Most times the best solution is the simplest.
Get a DC power light source and save yourself
a lot of work.

The hard way is to load the images into a stack,
reslice the stack such the the 60 Hz is displayed
in each frame.  The 60 Hz will appear as light and
dark bars (either vertical or horizontal, depending
on how you rotated the stack).  Filter out the bars
(probably FFT) and then rotate the stack back to its
original orientation.

Ray




Franklin Shaffer wrote:

> I'm taking high speed videos of particles at 10,000 frames per second, but the light source I'm using for illumination is varying in brightness at 60 hz.  
> The result is that the brightness of each image is different, even when the object being viewed is not changing.
>
> Anyone have any suggestions for image processing steps to correct for the varying illumination?
>
> (I don't have the option of getting a different light source.)
>
> Thank you,
>
> Frank Shaffer
> Research Engineer
> USDOE National Energy Technology Laboratory
> Computational Science Division
>