Re: Using ImageJ for electron probe micro analysis images

Posted by Gabriel Landini on
URL: http://imagej.273.s1.nabble.com/counting-pixels-of-one-color-or-color-range-tp3694997p3695000.html

On Monday 22 September 2008 22:35:02 Donald S. Ross wrote:

> Greetings,  I am new to this list.  We have 16-bit images giving
> elemental maps of soil particles from EPMA (electron probe micro
> analysis).  I've been trying out ImageJ for awhile and can do the
> basics of importing, thresholding and determining areas.  I need to
> get a little more advanced such as calculating the area of a certain
> range in ratios of elements (e.g. Si:Al and Si:Al:Ca) from two or
> three maps, but I'm stuck.  It seems as if someone should have done
> this before.  Does anyone know a good resource, i.e. tutorial or link
> with info on this type of application?  I've searched through the
> plugin list but haven't found anything yet.

Would this work?

Assuming that image intensity gives the amount of material (I have no idea about EPMA) then you could go testing pixel by pixel the ratio of intensity of the 2 or 3 images. Let's say you want a ratio of a:b between 0.2 and 0.5

Or, you could divide one 32 bit image by another (using the Calculator Plus) and threshold the result image between 0.2 and 0.5

G.