Re: Image quality evaluation

Posted by Emmanuele Sordini on Jul 12, 2006; 10:19am
URL: http://imagej.273.s1.nabble.com/Image-quality-evaluation-tp3695062p3695069.html

Hello Christian!

On 7/12/06, Breukers, C (TNW) <[hidden email]> wrote:
>
> Hello Jennifer,
>
> Normally we use a Signal-to-Background Ratio equation:
>
> ((Mean of Max. intensity)-(Mean of Background intensity))/ Standard
> Deviation of the background.


I would be interested in giving that indicator a try. However, I do have a
few questions:

1) How can you calculate the std. deviation of the background? i.e. how can
you tell the background from image subject (useful signal) and perform the
calculation only on the former? I'd rather take the std. deviation of the
whole image, which is of course close to that of the background if the
subject takes up only a fraction of the whole image.

2) For "Mean of max. intensity" and "mean of background intensity", see #1
as well. It all boils down to separating the signal and the background
correctly.

I would really appreciate it if you could elaborate a bit more on this
topic.

Emmanuele