Count cells in stack?
Posted by Mike Myerburg on Apr 11, 2008; 6:54pm
URL: http://imagej.273.s1.nabble.com/Count-cells-in-stack-tp3695075.html
Hi-
I study polarized cells that form a pseudostratified epithelial layer on
filters. I am trying to make a plugin that will count the # of nuclei
present in a stack. Because the cells are not a monolayer, I cannot
accurately count them by making an zprojection and then do standard
automated counting (the nuclei are occasionally on top of each other).
It seems that it "should" be possible to make a plugin to analyze the
nuclei in 3D and then count the number within a stack.
Has something like this already been made? Any suggestions?
Cheers, Mike