Re: Object-Image successor

Posted by Michael Schmid on Aug 25, 2008; 4:35pm
URL: http://imagej.273.s1.nabble.com/Object-Image-successor-tp3695259p3695260.html

Hi Håkan,

if it is good enough to have numbers, the built-in point
selection tool could help.

Double-click it and check "Label points".

You can get a point selection manually, in a macro (makePoint
command) or as a result of Process>Binary>Find Maxima.

Michael
________________________________________________________________

On 25 Aug 2008, at 17:34, Håkan Toresson wrote:

> My group does work on dynamic changes within dendritic spines. We thus
> analyse at least two channels; one for the spine and one or more  
> for an
> organelle or protein of interest.  The analysis is done manually  
> over time
> (from three time-points and up) by evaluating, spine by spine, the  
> content
> of different markers. Typically a stack will contain a few hundred  
> markers.
>
> For a long time we have been using Object-Image for these analyses.  
> As the
> number of MacOS9 computers go down this becomes a less convenient  
> solution
> though...
>
> The requirements for the software we need are some key features of  
> Object-Image.
> 1. That non-destructive labels can be added to time stacks (and  
> sometimes
> z-stacks) to mark the position of different classes of spines and,
> evidently, that the program records the number and position of the  
> different
> labels.
> 2. That a label set in one of the slices can be seen in all other  
> slices.
> This is important because we set one label for a feature observed  
> in one
> channel (e.g. stubby spine) and then another feature at a later  
> time-point
> (e.g. the presence of GFP-labelled CamKII).
>
> I’m new to ImageJ and would very much appreciate if anyone could  
> let me know
> of a suitable plugin for ImageJ to perform such analyses. I’ve  
> tried ObjectJ
> (created by Norbert Visscher who did Object-Image) but so far I’m  
> unable to
> perform what I need.
>
> Kind regards,
> HT